Journal: Cell Reports Medicine

Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

doi: 10.1016/j.xcrm.2025.101966

Figure Lengend Snippet:

Article Snippet: In addition, the cDNA sequences of E2F1 and RING1 gene were cloned into the pRK5-HA vector. pCMV3-His-Rb (#HG10137-NH) was purchased from Sino Biological Inc. (Beijing, China). pRK5-HA-Ubi, pRK5- Ring1 , pRK5- UBE2T , deletions-mutant p53 plasmids were constructed in our previous article.

Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software

Journal: Cell Reports Medicine

Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

doi: 10.1016/j.xcrm.2025.101966

Figure Lengend Snippet:

Article Snippet: In addition, the cDNA sequences of E2F1 and RING1 gene were cloned into the pRK5-HA vector. pCMV3-His-Rb (#HG10137-NH) was purchased from Sino Biological Inc. (Beijing, China). pRK5-HA-Ubi, pRK5- Ring1 , pRK5- UBE2T , deletions-mutant p53 plasmids were constructed in our previous article.

Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software

Journal: Cell Reports Medicine

Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

doi: 10.1016/j.xcrm.2025.101966

Figure Lengend Snippet:

Article Snippet: pCMV3-His-Rb , Sino Biological , HG10137-NH.

Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: (A) PC-3 cells infected with lentivirus for indicated shRNAs were treated with different chemicals, and cell viability was measured by MTS assay. Heatmap showing the normalized IC50 ratio (log2[IC50ratio]).

Article Snippet: lentivirus-expressing RB1-shRNAs (human) , Sigma-Aldrich , SHCLNG-NM_000321.

Techniques: Infection, MTS Assay

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: (A) RNA-seq analysis indicates the genes commonly upregulated by RB1 knockdown and palbociclib treatment in PC-3 cells.

Article Snippet: lentivirus-expressing RB1-shRNAs (human) , Sigma-Aldrich , SHCLNG-NM_000321.

Techniques: RNA Sequencing Assay

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: (A–C) PC-3 cells were infected with lentivirus expressing indicated shRNAs, and PD-L1 expression was determined by western blots (A), FACS (B), and IFC (C). (D and E) PC-3 cells infected with lentivirus expressing indicated shRNAs were harvested for western blotting (D) and RT-qPCR (E). All data are shown as mean values ± SD (n = 3). ns, not significant; ***p < 0.001.

Article Snippet: lentivirus-expressing RB1-shRNAs (human) , Sigma-Aldrich , SHCLNG-NM_000321.

Techniques: Infection, Expressing, Western Blot, Quantitative RT-PCR

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: (A and B) PC-3 cells infected with lentivirus expressing indicated shRNAs were treated with or without gamma radiation (IR, 12 Gy) followed by western blotting (A) and RT-qPCR (B). All data are shown as mean values ± SD (n = 3). ns, not significant; **p < 0.01.

Article Snippet: lentivirus-expressing RB1-shRNAs (human) , Sigma-Aldrich , SHCLNG-NM_000321.

Techniques: Infection, Expressing, Western Blot, Quantitative RT-PCR

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: lentivirus-expressing RB1-shRNAs (human) , Sigma-Aldrich , SHCLNG-NM_000321.

Techniques: Recombinant, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software